1888 - Roundhay Garden Scene
Roundhay Garden Scene (1888) was shot by Louis Aime Augustin Le Prince using the Le Prince single-lens camera. It lasts for less than 2 seconds and includes 4 frames. http://www.imdb.com/title/tt0392728/
From the IMDB plot summary: “It lasts for less than 2 seconds and includes 24 frames.”